The U.S. Army needs more horses for the Spanish-American War. Hoppy must turn his Bar 20 cowhands into Rough Riders to gather up the horses, and of course bad guys try to sabotage the operation.
Burton is after Clark's ranch. He gets the banker to refuse to renew Clark's note and then sends his men to rustle his cattle. Hoppy is Clark's new foreman and is on to Burton's scheme. But just as he learns of the rustling and is about to go after the gang, the Sheriff arrives and arrests him for hiding Johnny who has been accused of robbery.
Finishing a trail drive, Hoppy and the boys head to town and immediately get caught up in the conflict between school teacher Miss Abott and next door saloon owner Mawson. When Miss Abott disappears, Hoppy gets a clue to her location and rescues her from Mawson's cabin. It looks like Mawson is the man he wants, but Hoppy finds an item that indicates otherwise.
Before he was killed by Mark Foster's men, Bud Lawton willed part ownership in his ranch to Hoppy and his two pals. When the three arrive they find a fake posing as Lawton. When they expose the imposter, Foster gets the Sheriff to jail them for Lawton's murder.
Hoppy is an agent of the federal government investigating the counterfeiting of both U.S. and Mexican money. His only clues are the name "Mordigan" and a drawing of a comet.
Escaped criminal "The Fox" hates Hoppy and a Rurales colonel for imprisoning him and lures Cassidy to Mexico in order to exact his vengeance.
A town bedeviled with outlaws sends for Hoppy, Lucky and California after their own vigilante committee fails to solve the towns problems. Hoppy discovers that the bad guys are led by the town boss, and so are the vigilantes.
While oil drilling, Hoppy and California discover an underground well - a potential threat to Jebb Hardin's monopoly on water in the region.